This page was last updated on Friday, 08-Jan-2016 17:17:14 CST

Installation notes for Trinotate version 2.0.2

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Prerequisites

• Trinity
• sqlite
  $ sudo apt-get install sqlite
• NCBI BLAST+
• HMMER/PFAM

Installation

1. $ ver="2.0.2"
2. $ cd /programinstallers/
3. $ wget -N https://github.com/Trinotate/Trinotate/archive/v$ver.tar.gz
4. $ mv v$ver.tar.gz Trinotate-$ver.tar.gz
5. $ cd /usr/local/bin
6. $ tar -zxvf /programinstallers/Trinotate-$ver.tar.gz
7. $ cd Trinotate-$ver
8. Make a version-independent symlink
   $ cd /usr/local/bin && ln -s Trinotate-$ver trinotate
9. $ cd trinotate
10. Retrieve the Trinotate Pre-generated Resource SQLite database
    $ wget "https://data.broadinstitute.org/Trinity/Trinotate_v2.0_RESOURCES/Trinotate.sprot_uniref90.20150131.boilerplate.sqlite.gz" -O Trinotate.sqlite.gz
11. 5.8G when uncompressed
    $ gunzip Trinotate.sqlite.gz
12. $
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