Doug Senalik's Blog — Things that aren't on the internet yet

2010

⚙ Blast2GO notes

☷ December 11, 2010 ☷

Some random notes about Blast2GO:
To get around this error message in Blast2GO:

Error in blast result (XML has over 6000 lines
Run the BLAST step again, but reduce the number in the "Number of Blast Hits" box.

☷ path: /2010permanent link to this entry

⚙ OpenWRT connection to UW VPN

☷ October 11, 2010 ☷

We have some WRT54GL routers running OpenWRT Linux, and modified to record temperatures with 1-Wire sensors in the greenhouse. There is no wired network there, so we need to use the UW Wireless network that is available. There is a hard to find page with information about setting up WiscVPN on linux here: http://kb.wisc.edu/helpdesk/page.php?id=9120

But when connecting to DoIT VPN, the connection drops after about 30 minutes, and cannot be reconnected for another 45-60 minutes.

The connection drops when DHCP renewal occurs, and appears to only work again when the DPD (Dead peer detection) times out and declares the peer dead. I say this because if you disable dead peer detection, you are locked out for the entire day.

This web page had an answer: http://svn.dd-wrt.com:8000/dd-wrt/ticket/973

Add this firewall rule to /etc/firewall.user

# Rule to prevent VPN dropping when DHCP is renewed
iptables -I INPUT -p udp --dport 68 -j ACCEPT

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⚙ Installing Roche 454 Analysis software (gsAssembler and gsMapper) on Fedora 13

☷ October 2, 2010 ☷

In case it helps anyone else, to install the Roche 454 sequence analysis software on a computer running 64 bit Fedora 13, there were a few libraries that needed to be installed first:

glibc.i686 zlib.i686 libXi.i686 libXtst.i686 libXaw.i686

Roche software version was 2.3

So these two commands took care of that (I could have combined them into one of course):

$ sudo yum install glibc.i686
Installed:
  glibc.i686 0:2.12-3

Dependency Installed:
  nss-softokn-freebl.i686 0:3.12.6-3.fc13

Dependency Updated:
  glibc.x86_64 0:2.12-3
  glibc-common.x86_64 0:2.12-3
  glibc-devel.x86_64 0:2.12-3
  glibc-headers.x86_64 0:2.12-3

$ sudo yum install zlib.i686 libXi.i686 libXtst.i686 libXaw.i686
Installed:
  libXaw.i686 0:1.0.6-4.fc12
  libXi.i686 0:1.3-2.fc13
  libXtst.i686 0:1.0.99.2-3.fc12
  zlib.i686 0:1.2.3-23.fc12

Dependency Installed:
  libICE.i686 0:1.0.6-2.fc13
  libSM.i686 0:1.1.0-7.fc12
  libX11.i686 0:1.3.1-3.fc13
  libXau.i686 0:1.0.5-1.fc12
  libXext.i686 0:1.1.2-2.fc13
  libXmu.i686 0:1.0.5-2.fc13
  libXpm.i686 0:3.5.8-2.fc13
  libXt.i686 0:1.0.7-1.fc13
  libuuid.i686 0:2.17.2-7.fc13
  libxcb.i686 0:1.5-1.fc13

Dependency Updated:
  libblkid.x86_64 0:2.17.2-7.fc13
  libuuid.x86_64 0:2.17.2-7.fc13
  util-linux-ng.x86_64 0:2.17.2-7.fc13

☷ path: /2010permanent link to this entry

⚙ Obscure Useful Information

☷ August 16, 2010 ☷

How to edit /etc/fstab when at Fedora "Repair filesystem" prompt?
Source: http://www.techiegyan.com/?p=86

Use following command to mount the filesystem with writable permission:

Repair filesystem # mount -w -o remount /

☷ path: /2010permanent link to this entry

⚙ Eppenorf Mastercycler Repair

☷ February 4, 2010 ☷

Keywords: power failure
Did your Eppendorf Mastercycler Gradient 5331 suddenly go dead? And it isn't the fuse? And it IS plugged in?

Two of ours have failed in this manner, but the repair costs only about $10.
For the benefit of anyone who may have this same problem, it was the inrush current limiter.

This image shows the location of the current limiter, here labeled SL15 20004 (Amtherm, Inc.)
Before repair

A closeup
Before repair - detail

This part was not available, I replaced it with a MS22 20005, Digi-Key part number 570-1007-ND

After repair

☷ path: /2010permanent link to this entry

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