USDA ARS VCRU Data Server This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page] |
Return to B493×QAL v.1 map main page
or to BSB×HCM map main page
The data used in making this map might be in these Microsoft Excel files
full 493 x qal1.xls 1/31/2002 12:45 pm
(Note that the marker GGGCAA097-Q was in this file twice, with different scores, I have renamed them to GGGCAA097-Q[1] and GGGCAA097-Q[2])
bsb x hcm 1-145.xls 1/31/2002 12:13 pm
bsb x hcm146-291.xls 1/31/2002 12:13 pm
The most complete single file for 493×QAL, a consolidated copy with ALL data for ALL markers up to Darek's map, is here
A recoding of the bsb x hcm to AC and BD format bsb x hcm-all.xls
File emailed by Carlos with 289 markers. In his thesis was reported 287 markers. mappingdata.txt
And this file was converted to microsoft excel = mappingdata.xls
Hand entered from the figures (watch for mistakes),
a text file with distances and markers is
BSBxHCMmap.txt
B493xQALmap.txt
Table 3.1 Overview of EcoRI/MseI and PstI/MseI AFLP primer combinations, numbers of screened and assigned AFLP fragments to nine linkage groups of two F2 populations of carrot
Primer combination | Brasilia × HCM | B493 × QAL | ||
Screened | Assigned to Linkage Group | Screened | Assigned to Linkage Group | |
EAAC MCAA | 10 | 8 | - | - |
EAAC MCAC | 5 | 1 | 6 | 2 |
EAAC MCAT | 21 | 17 | 20 | 16 |
EAAC MCTA | 5 | 4 | 3 | 3 |
EAAG MCAG | 14 | 7 | 18 | 13 |
EAAG MCTA | 11 | 10 | - | - |
EAAG MCTC | 14 | 6 | 19 | 12 |
EAAG MCTT | 13 | 6 | - | - |
EACA MCAA | 17 | 13 | 18 | 13 |
EACA MCTT | 11 | 8 | 19 | 15 |
EACC MCAC | 15 | 9 | 10 | 7 |
EACC MCAG | 10 | 6 | 3 | 0 |
EACC MCTA | 13 | 7 | 10 | 10 |
EACC MCTG | 6 | 4 | 4 | 4 |
EACG MCAT | 17 | 10 | 1 | 1 |
EAGC MCAA | 12 | 7 | 12 | 6 |
EAGC MCTT | 12 | 9 | 14 | 10 |
EAGG MCAT | 12 | 9 | 14 | 7 |
EAGG MCTA | 10 | 7 | 14 | 12 |
EAGG MCTG | - | - | 7 | 6 |
PGAG MCAC | 24 | 17 | 5 | 5 |
PGAG MCTT | 16 | 14 | 15 | 13 |
PGAT MCAT | 28 | 21 | 5 | 5 |
PGAT MCTC | 14 | 8 | 16 | 13 |
PGGA MCAA | 14 | 12 | 10 | 8 |
PGGA MCAG | 16 | 11 | 16 | 16 |
PGGG MCAA | 12 | 7 | 16 | 14 |
PGGG MCAT | 17 | 13 | 14 | 10 |
PGGG MCTG | 23 | 16 | 9 | 9 |
PGGT MCAG | 15 | 10 | 14 | 11 |
PGGT MCTT | 14 | 10 | 12 | 12 |
Total | 421 | 287 | 318 | 250 |
Table 3.2 Evaluation of Brasilia × HCM and B493 × QAL F2 populations for primers and resolution systems used to screen a series of molecular markers, which have been reported in the literature as giving co-dominant information in carrot
Marker | Primer (5’ → 3’) | Resolution | Size | Co-dominant information | Other Information |
---|---|---|---|---|---|
1- Niemann et al. (1997) |
|||||
DCEXTG | ACACCGACTCTGAAAAGTAA | Acrylamide/Agarose | 180 | No | (TTTC)3TTTT cDNA, extensin |
GCCAACAAGTCAACAACTAT | |||||
DARKUROAI | TTTTGGAGTGAGTGATGGGTCTG | Acrylamide/Agarose | 201 | No | (TC)4CC(TC)6 cDNA |
GGTTTTTTCAAGGGGAGAGAGAG | |||||
DARKUROAII | CACCCTTCTTTCAGCTCTCTC | Acrylamide/Agarose | 229 | No | (TC)10 cDNA |
GAAACTCAGAAGAGGGTGGGTG | |||||
DCHSP70 | TTCTCTCAGCTCCTCTGTTT | Acrylamide/Agarose | 169 | No | (AACA)4 cDNA, heatshock-protein |
GCTAGCCATCGTGATCAGTT | |||||
DARGCHS2 | CAACCGGACAGATGGCTATT | Acrylamide/Agarose | 203 | No | (TTAT)3CGCTTAT cDNA, chalcone synthase |
CCGGAACTCATTCACAGTCA | |||||
DCDRTSG | GAATGTGCGATTATCTCTCTCG | Acrylamide | 130 | Yes | (TC)9 cDNA, dihydrifolate reductase-thymidylate synthase transitpeptide |
AATTGACGGCAGAAAAGGTG | |||||
DCU47081 | CATCCTCACCACCACAAGC | Acrylamide/Agarose | 241 | No | (CAC)5AAACAC cDNA,somatic embryogenesis |
TGCAGGGCTTGCAGTAGAC | |||||
DCU47090 | TTAGTAAAAAACCGTTGCTGTCG | Acrylamide/Agarose | 111 | No | T(18) cDNA,somatic embryogenesis |
ATATTGTGCCCCAGCTTTTG | |||||
DCU47097 | TGCTGGATTTGGAGTAGGCT | Acrylamide/Agarose | 194 | No | (CAG)6(GTG)2(GAG)7 cDNA,somatic embryogenesis |
TAGGGAGTGGAATGTTTTTTCTG | |||||
DCU62395 | TGTTTGATTCAATGTTTTGCCCTG | Acrylamide/Agarose | 201 | No | (TTTC)5TTTT cDNA, globuline-like protein |
GCGTTGTTCTCAAGATCCATTTCA | |||||
2 - Boiteux (2000) |
|||||
SQ1850/SQ1700 Protocol MK000003.pdf |
GGACGATGGCCAGGGAAAGC | Acrylamide/Agarose | 700 / 850 | No | Also known as Q1800 |
AACCAAGTCACGCCAACAGTAATT | |||||
SQ6500/SQ6590 Protocol MK000009.pdf |
AGCGCCTTGATTGATGCTGTGTTGCC | Acrylamide/Agarose | 500 / 590 | No | Also known as Q6500 or Q6 TIPICO |
GAGCGCCTTGGCAGCATCGATTAGTAG | |||||
3 - Bradeen and Simon (1998) |
|||||
Y2 locus Protocol MK000002.pdf |
TAAAGTCGTATAGGAAGAACAT | Acrylamide/Agarose | 172 / 310 | No | |
TGGATCATCAGAACTCAACT | |||||
4 - RAPD Vivek (1997) |
|||||
SAMPL (4) | (List from Vivek) | Acrylamide | No | ||
OP-K9C Protocol MK000054.pdf |
(transformed to SCAR) | Agarose | ? | Yes–493xQAL | |
5 - Inga Bach (unpublished) |
|||||
SSR-W93 Protocol MK000055.pdf |
ATAATAGGGCGTTTCTTGTTCTT | Agarose | 285 and ? | Yes–493xQAL | |
CGGGTAGGCTTAATGTTCTGT |