USDA ARS VCRU Data Server
This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page]
 

Brasilia 1252×B6274 RAPD Linkage Map

This map was published in Characterization of the Meloidogyne javanica resistance locus employing molecular markers and isolation of candidate disease resistance loci in the carrot (Daucus carota L.) genome
by Leonardo Silva Boiteux, Ph.D. Thesis, 2000, University of Wisconsin-Madison
Chapter 8: Construction of an RAPD-based genomic map of carrot with anchor sequence-tagged site markers corresponding to microsatellite repeats, retrotransposon elements, promoter-like and putative coding regions, Tables 8.1 through 8.24

Details about the markers

Details about individual markers are available by clicking on any of the markers on the map, or by going directly to the marker information page

Linkage map

RAPD linkage groups of the female parent ‘Brasilia-1252’ estimated via analysis of 112 F2 individuals from the cross ‘Brasilia-1252’ × ‘6274B’. Genetic distances between adjacent markers were estimated using Kosambi function.
(Figures 8.1 through 8.12)

Linkage                     Operon  Approximate  Genetic
  Group                     Primer    size (bp) distance   Total

       1                     OP-C6          1100     24.8
       1                    OP-U17          1500      9.2
       1                     OP-C9          1600     23.9
       1                    OP-F14          1050     ---     57.9

       2                     OP-A4           720      8.3
       2                    OP-B11          1300     16.5
       2                    OP-AA4          1500     12.7
       2                    OP-C16          1300      2.1
       2                     OP-C6          2070     12.0
       2                     OP-C8          1200     19.8
       2                     OP-Y7           300     40.1
       2                     OP-V3           850     ---    111.4

       3                     OP-C6          2000     12.3
       3                    OP-C20           850      4.2
       3                     OP-D2          1250     17.6
       3                     OP-E9          1500     ---  34.1cM

       4                     OP-H4          1050     19.0
       4                    OP-C20           980      9.2
       4                    OP-H14           900     16.9
       4                    OP-J12          1600     10.2
       4                    OP-Q18           750     18.3
       4                     OP-N5          1000     52.6
       4                    OP-N14           750      6.4
       4                    OP-B11           850      4.5
       4                     OP-D8          1300      3.1
       4                    OP-A17          1800      5.5
       4                     OP-C8          1700     --- 145.7cM

       5                     OP-J4          1050      9.4
       5                     OP-E7           950     30.8
       5                    OP-F12           350      6.1
       5                     OP-D8          1600      1.4
       5                    OP-H14          1100      3.3
       5                    OP-W12           300      8.0
       5                     OP-W2           420     19.5
       5                    OP-B12           800     ---  78.4cM

       6                     OP-H4          1900     15.6
       6                     OP-P4           700      5.3
       6                     OP-K8           550     22.6
       6                    OP-J10           800      5.6
       6                    OP-X16           650      4.3
       6                     OP-H8          1000     17.0
       6                     OP-F6           400     10.6
       6                    OP-K14          1100     ---  81.0cM

       7                    OP-F12           750      9.5
       7                     OP-A4           300     26.4
       7                    OP-H20          1400      4.0
       7                      Mj-1          ---       5.1
       7                     OP-D2           750     ---  45.0cM

       8                    OP-H11           400     18.8
       8                     OP-D8           720     23.8
       8                     OP-J4          1000     ---  42.6cM

       9                     OP-C8          1300      6.0
       9                     OP-C9          2100     ---   6.0cM

      10                     OP-D2           920     22.6
      10                     OP-K4           900     ---  22.6cM

      11                     OP-F8          1600      7.4
      11                    OP-K14          1400     ---      7.4

      12                    OP-K10           850      4.4
      12                    OP-L12           800     ---   4.4cM

RAPD linkage groups of the male parent ‘6274B’ estimated via analysis of 112 F2 individuals from the cross ‘Brasilia-1252’ × ‘6274B’. Genetic distances between adjacent markers were estimated using Kosambi function.
(Figures 8.13 through 8.24)

Linkage                     Operon  Approximate  Genetic
  Group                     Primer    size (bp) distance   Total

       1                    OP-H12          2070     23.1
       1                    OP-K14          1400      6.2
       1                     OP-C8          1250     25.9
       1                     OP-N2          1150     ---  55.2cM

       2                    OP-A17          1500      8.1
       2                     OP-Q5           700     17.5
       2                     OP-F8          1300     ---     25.6

       3                     OP-K8          1100      4.7
       3                     OP-P2           750     28.8
       3                    OP-H11          1850      3.9
       3                    OP-B12          1100      1.8
       3                    OP-A17          1200     10.0
       3                    OP-H11          2100     10.0
       3                    OP-A17          1850     52.0
       3   mj-1(susceptible locus)          ---       0.0
       3                     OP-Q1           850      0.9
       3                    OP-J12          2200      4.5
       3                    OP-H11          1600      0.0
       3                     OP-F6          1200      5.6
       3                     OP-E7          1500      3.2
       3                     OP-D8           650      4.9
       3                     OP-D2          1030     --- 130.4cM

       4                    OP-Q13           400     28.1
       4                    OP-W19          1200     16.2
       4                    OP-V13           580      4.2
       4                     OP-F8          1500     13.5
       4                     OP-K3           900     ---  62.0cM

       5                     OP-D8           800      6.1
       5                    OP-F12           850     17.0
       5                     OP-S7          1000     15.8
       5                     OP-T6          1300     15.4
       5                     OP-D2          1150     ---  54.3cM

       6                     OP-J6           800     18.7
       6                    OP-O12           600     19.1
       6                    OP-H20           790     30.8
       6                    OP-C10           900     10.7
       6                     OP-H9          1200     ---  79.3cM

       7                     OP-I3          1100     25.0
       7                    OP-E14           700      3.2
       7                     OP-H4          1100     22.9
       7                    OP-K14           700     ---  51.1cM

       8                    OP-E14           900      5.8
       8                    OP-K10          1200     21.1
       8                    OP-N10          1100     ---  26.9cM

       9                    OP-W17           800     15.3
       9                     OP-F6          1420     ---  15.3cM

      10                    OP-Q15           700      3.2
      10                     OP-B7           800     ---   3.2cM

      11                    OP-B17           700     12.0
      11                     OP-I7           290     ---  12.0cM

      12                     OP-H2          1250     23.9
      12                     OP-H8           550     ---  23.9cM