USDA ARS VCRU Data Server
This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page]
 

Brasilia 1252×B6274 AFLP Linkage Map

This map was published in Characterization of the Meloidogyne javanica resistance locus employing molecular markers and isolation of candidate disease resistance loci in the carrot (Daucus carota L.) genome
by Leonardo Silva Boiteux, Ph.D. Thesis, 2000, University of Wisconsin-Madison
Chapter 6: Molecular Tagging of the Meloidogyne javanica Resistance Locus with Amplified Fragment Length Polymorphism (AFLP) Markers and Sequence Characterization of AFLP Loci in Carrot (Daucus carota)

Details about the markers

Details about individual markers are available by clicking on any of the markers on the map, or by going directly to the marker information page

Linkage map

Linkage relationships between the Mj-1 locus and AFLP and AFLP-derived markers were estimated using MAPMAKER version 3.0 for IBM-PC (Lander et al., 1987; Lincoln et al. 1992). Linkage groups were first assigned based upon two-point analysis via ‘group’ command. Markers were initially associated with two-point comparisons of the group command (threshold LOD score = 5.0 and recombination fraction 0.25). The most likely order of the markers was obtained using exhaustive multipoint analysis via ‘compare’ command and the final maximum likelihood map was obtained with ‘map’ command. Recombination frequencies were transformed to Kosambi map distances (Kosambi, 1944).

Figure 6.2. The AFLP linkage map of the Mj-1 (Meloidogyne javanica resistance) locus in carrot. The map population was composed by 188 F2 individuals from the cross ‘Brasilia-1252’ (resistant parent) × ‘6274B’ (susceptible parent). The genetic distance in cM (Kosambi function) between pairs of markers is shown at the left and the AFLP marker designation is shown on the right

P7B9Z
P5B6Z
P4B5Z
P6B6U
Mj-1
P3B3Z
P8B8U
P4B2U
P8B3Z
P6B11U
P5B11U