36th International Carrot Conference Abstract

Mapping genes important for flower architecture and pollen development in carrot (Daucus carota L.)

Thomas Nothnagel¹, Bettina Linke⁴, Rafał Barański³, Dariusz Grzebelus³, Agnieszka Kiełkowska³, Petra Straka², Kai Metge² and Holger Budahn¹

¹Institute for Breeding Research on Horticultural and Fruit Crops, ²Institute for Biosafety in Plant Biotechnology, Federal Research Centre for Cultivated Plants, Julius Kühn-Institute (JKI), Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany

³Dept. of Genetics, Plant Breeding and Seed Science, Faculty of Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425 Kraków, Poland

⁴Department of Biology, Humboldt University Berlin, Invalidenstr. 42, D-10115, Berlin, Germany

A linkage map of carrot was developed with main focus on genes involved in flower architecture and pollen development. An F₂ mapping population (n=161) was created by an initial cross between the two genetically well described carrot mutants - the chlorophyll mutant yellow leaf (yel) and the semi-dwarf mutant compressed lamina (cola). Four distinct phenotype classes were observed in the segregating F₂ progeny as expected for two independent genes. Variations in flower architecture and pollen development, such as epigynous (wild type) flowers with normal pollen fertility and hypogynous (mutant) flowers with reduced pollen fertility, were associated with each of the two phenotype classes. The genetic map constructed had a total length of 806 cM and included 288 markers. The length of the nine linkage groups ranged from 60 cM to 143 cM. The linkage groups were anchored to the reference map using 18 SSRs, 2 SCARs and 1 EST marker. The loci YEL and COLA were assigned to the linkage groups 1 and 4, respectively. Two members of the MADS-box gene family, DcMADS3 (B-class) and DcMADS5 (E-class), with prominent roles in flowering and reproduction were mapped to the linkage groups 5 and 7, respectively. Additionally, three genes (DcAOX2a, DcAOX2b and DcCHS2) with further importance for male reproduction were localised on the linkage groups 6 and 9.

Last updated Thursday, 25-Jul-2013 11:53:13 CDT