You must specify a batch ID and it must be an integer (e.g. 1, 2, 3). ref_map.pl 1.35 ref_map.pl -p path -r path [-s path] -o path [-n mismatches] [-m min_cov] [-T num_threads] [-A type] [-O popmap] [-B db -b batch_id -D "desc" -a yyyy-mm-dd] [-S -i id] [-e path] [-d] [-h] p: path to a Bowtie/SAM/BAM file containing parent sequences from a mapping cross. r: path to a Bowtie/SAM/BAM file containing progeny sequences from a mapping cross. s: path to a Bowtie/SAM/BAM file containing an individual sample from a population. o: path to write pipeline output files. A: if processing a genetic map, specify the cross type, 'CP', 'F2', 'BC1', 'DH', or 'GEN'. n: specify the number of mismatches allowed between loci when building the catalog (default 1). T: specify the number of threads to execute. m: specify the minimum depth of coverage to report a stack in pstacks (default 1). O: if analyzing one or more populations, specify a pOpulation map e: executable path, location of pipeline programs. d: perform a dry run. Do not actually execute any programs, just print what would be executed. h: display this help message. Database options: B: specify a database to load data into. b: batch ID representing this dataset in the database. D: batch description a: batch run date, yyyy-mm-dd S: disable recording SQL data in the database. i: starting sample_id, this is determined automatically if database interaction is enabled. SNP Model Options (these options are passed on to pstacks): --bound_low : lower bound for epsilon, the error rate, between 0 and 1.0 (default 0). --bound_high : upper bound for epsilon, the error rate, between 0 and 1.0 (default 1). --alpha : chi square significance level required to call a heterozygote or homozygote, either 0.1, 0.05 (default), 0.01, or 0.001. Arbitrary command line options: -X "program:option": pass a command line option to one of the pipeline components, e.g.'-X "sstacks:-x"'.