You must specify an input file of a supported type. pstacks 1.35 pstacks -t file_type -f file_path [-o path] [-i id] [-m min_cov] [-p num_threads] [-h] t: input file Type. Supported types: bowtie, sam, or bam. f: input file path. o: output path to write results. i: SQL ID to insert into the output to identify this sample. m: minimum depth of coverage to report a stack (default 1). p: enable parallel execution with num_threads threads. h: display this help messsage. Model options: --model_type : either 'snp' (default), 'bounded', or 'fixed' For the SNP or Bounded SNP model: --alpha : chi square significance level required to call a heterozygote or homozygote, either 0.1, 0.05 (default), 0.01, or 0.001. For the Bounded SNP model: --bound_low : lower bound for epsilon, the error rate, between 0 and 1.0 (default 0). --bound_high : upper bound for epsilon, the error rate, between 0 and 1.0 (default 1). For the Fixed model: --bc_err_freq : specify the barcode error frequency, between 0 and 1.0.