You must specify an input file of a supported type. estacks 1.35 estacks -t file_type -f file_path [-o path] [-i id] [-m min_cov] [-r] [-e errfreq] [-p num_threads] [-h] p: enable parallel execution with num_threads threads. t: input file Type. Supported types: bowtie, sam. f: input file path. o: output path to write results. i: SQL ID to insert into the output to identify this sample. O: record homozygotes along with heterozygote SNPs. m: minimum depth of coverage to report a stack (default 1). e: specify the barcode error frequency (0 < e < 1) if using the 'fixed' model. h: display this help messsage.