PRINSEQ-graphs v0.6 HTML Report   

Input Information

Input file(s):	example1.fastq
Input format(s):	FASTQ
# Sequences:	12
Total bases:	1,150

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Length Distribution

Mean sequence length:	95.83 ± 37.96 bp
Minimum length:	50 bp
Maximum length:	200 bp
Length range:	151 bp
Mode length:	100 bp with 8 sequences

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GC Content Distribution

Mean GC content:	48.17 ± 14.83 %
Minimum GC content:	0 %
Maximum GC content:	57 %
GC content range:	58 %
Mode GC content:	50 % with 5 sequences

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Base Quality Distribution

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Occurence of N

Sequences with N:	2  (16.67 %)
Max percentage of Ns per sequence:	10 %

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Poly-A/T Tails

	5'-end	3'-end
Sequences with tail:	1  (8.33 %)	1  (8.33 %)
Maximum tail length:	50	50

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Tag Sequence Check

	5'-end	3'-end
Probability of tag sequence:	33 %	33 %
GSMIDs or RLMIDs:	none

																					...
	1				5					10					15					20		20					15					10					5				1
	Position from Sequence Ends

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Sequence Duplication

	# Sequences	Max duplicates
Exact duplicates:	4  (33.33 %)	3
Exact duplicates with reverse complements:	0	0
5' duplicates	0	0
3' duplicates	0	0
5'/3' duplicates with reverse complements	0	0
Total:	4  (33.33 %)	-

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Sequence Complexity

	Value	Sequence
Minimum DUST score:	2	NNNNNNNNNNTACACCAGAGGTGTCTCTGTGTGGGGCCTGTGTGCCAAAAGTGAGAGTTG AGAAGAGGCGTGGAGGAGATGACACACCCCGTGTGTTCTC
Maximum DUST score:	100	AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Minimum Entropy value:	0	AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Maximum Entropy value:	81	NNNNNNNNNNTACACCAGAGGTGTCTCTGTGTGGGGCCTGTGTGCCAAAAGTGAGAGTTG AGAAGAGGCGTGGAGGAGATGACACACCCCGTGTGTTCTC

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Dinucleotide Odds Ratios

	AA/TT	AC/GT	AG/CT	AT	CA/TG	CC/GG	CG	GA/TC	GC	TA
Odds ratio	0.3954	2.4378	0.4858	0.3623	1.0736	0.3006	1.9166	0.4338	0.5829	1.8332