--------------------------------------------------------------------- Usage: MEGA-CC.exe -a /pathTo/analysisFile.mao -d /pathTo/dataFile.meg [-t /pathTo/treeFile.nwk][OPTIONS] -a --analysisOptions MEGA Analysis Options File *required* Specify the full path to the Mega Analysis Options (.mao) file. This file tells MEGA-CC which analysis to perform as well as which options to use -c --calibration Calibration file *optional* Specify the full path to a calibration file that you wish to use. The calibration file is used to provide calibration data for tree timing methods. -d --data Data File *required* Specify the full or relative path to the data file you wish to analyze. MEGA (.meg), and Fasta files are supported for all analyses. For distance matrices the MEGA (.meg) format is required. -f --format *applies to sequence alignment only* Export format for sequence alignment Sequence alignments can be exported in either the native .meg or FASTA format. Format values: MEGA Fasta -g --groups Groups file *optional* Specify the full path to the groups file that you wish to use. This file organizes taxa into groups where each line in the file is a key value pair of the form taxonName=groupName Group information is used for certain analyses, for instance, specifying which taxon/taxa comprise the outgroup for the timetree analysis -h --help Help Prints this help file document -n --noSummary: Do not write out the analysis summary file By default a file that gives an analysis summary is written. This option suppresses the export of that file. However, if any important messages are generated by the application, they will be written to this file regardless. -o --outfile *optional* Output Path / Output Dir Specify the full path and base filename (e.g. /myResultsDirectory/myResultName) or simply the full path and directory of where to save the file (e.g. /myResultsDirectory) in which case, a unique filename will be chosen automatically for you. -r --recursive Recursive directory search *optional* If a directory is specified for analysis by default MEGA only searches the contents of that folder and not any of it's children. To include the contents of all folders under the one specified, use this option. -s --silent: Do not write out the progress updates This option prevents progress updates from being written to stdout. -t --tree *required for some analyses* Tree File Specify the full path to the tree file you wish to use. (Some analyses requires a user provided tree, or allow you to provide your own) If no output path is specified, results will be saved in the same directory as the input data file, with a unique name.