Usage: deconseq [options] -f -dbs -dbs_retain ... Options: -help | -h Prints the help message and exists. -man Prints the full documentation. -version Prints the version of the program. -show_dbs Prints a list of available databases. -f Input file in FASTA or FASTQ format that contains the query sequences. -dbs Name of database(s) to use (default: hsref). Names are according to their definition in the config file. Separate multiple database names by comma without spaces. Example: -dbs hs1,hs2,hsref -dbs_retain Name of database(s) to use for cross-check. Query sequences with hit against any dbs will be compared to these databases. Databases have to be different from names in dbs. Names are according to their definition in the config file. Separate multiple database names by comma without spaces. Example: -dbs_retain bact,vir -out_dir Directory where the results should be written (default: .). If the directory does not exist, it will be created. -i Alignment identity threshold in percentage (integer from 1-100 without %) used to define matching sequences as similar. The identity is calculated for the part of the query sequence that is aligned to a reference sequence. For example, a query sequence of 100 bp that aligns to a reference sequence over the first 50 bp with 40 matching positions has an identity value of 80%. -c Alignment coverage threshold in percentage (integer from 1-100 without %) used to define matching sequences as similar. The coverage is calculated for the part of the query sequence that is aligned to a reference sequence. For example, a query sequence of 100 bp that aligns to a reference sequence over the first 50 bp with 40 matching positions has an coverage value of 50%. -group If dbs_retain is set, then this option can be used to group the sequences similar to dbs and dbs_retain databases with either the clean or the contamination output file. If group is not set and dbs_retain is set, then three separate files will be generated. Use -group 1 for grouping "Clean + Both" and use -group 2 for grouping "Contamination + Both". -no_seq_out Prevents the generation of the fasta/fastq output file for the given coverage and identity thresholds. This feature is e.g. useful for the web-version since -i and -c are set interactively and not yet defined at the data processing step. -keep_tmp_files Prevents from unlinking the generated tmp files. These usually include the id file and the .tsv file(s). This feature is e.g. useful for the web-version since .tsv files are used to dynamically generate the output files. -id Optional parameter. If not set, ID will be automatically generated to prevent from overwriting previous results. This option is useful if integrated into other tools and the output filenames need to be known. -S Chunk size of reads in bp as used by BWA-SW (default: 10000000). -z Z-best value as used by BWA-SW (default: 1). -T Alignment score threshold as used by BWA-SW (default: 30).